FvWRKY50 is an important gene that regulates both vegetative growth and reproductive growth in strawberry

Abstract WRKY transcription factor plays important roles in plant growth and resistance, but only a few members have been identified strawberry. Here we FvWRKY50 diploid strawberry which played essential vegetative reproductive growth. Knocking out by genome editing accelerated flowering time leaf senescence delayed anthocyanin accumulation fruit. Further analysis showed that acted as transcriptional repressor to negatively regulate the expression of related genes including FvFT2, FvCO, FvFT3 FvSAUR36. Notably, directly upregulated FvCHI FvDFR binding their promoter under normal condition, at low temperature was phosphorylated FvMAPK3 then induced protein degradation ubiquitination, delaying accumulation. In addition, homozygous mutant smaller while biallelic size. These new findings provide clues for us further reveal regulatory mechanisms fruit ripening.